Coding

Part:BBa_K2207036:Design

Designed by: Shisheng Li   Group: iGEM17_ZJU-China   (2017-10-23)


oligoA-mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 561
    Illegal AgeI site found at 673
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This oligoA should directly link with the start codon and the interruption may ruin its function.


Source

The sequence of this part is mainly from BBa_E1010, and we adapted PCR to optimize it.

References